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Cardiovascular health: from cardiomyocyte electrostimulation to miRNA detection

Ó Maolmhuaidh, Fionn orcid logoORCID: 0000-0001-5455-7611 (2023) Cardiovascular health: from cardiomyocyte electrostimulation to miRNA detection. PhD thesis, Dublin City University.

Abstract
Current methods of cell culture where electrical stimulation is applied during culture require a wired connection to a power supply to generate an electric field with which to stimulate the cells. This method is intrusive in a lab setting and does not conveniently allow for traditional cell culture techniques during stimulation, hence it is frequently omitted from cell culture protocols. The aim of this work is to demonstrate a novel method of electrical stimulation of cardiomyocytes using wireless bipolar electrochemical techniques. The work describes the design and characterisation of a wireless bipolar electrode and wireless bipolar electrochemical cell to facilitate wireless bipolar electrostimulation. By using a wireless connection more versatile experiments can be conducted on cells in culture while mitigating the contamination risk of a traditional wired stimulation platform. Using a polypyrrole based conducting film doped with fibronectin molecules to facilitate the adherence and growth of cardiomyocytes on the bipolar electrode surface. Cell culture on a conductive film opens the possibility of future applications in electroceuticals by providing a wireless platform to deliver and electric field to cells in culture. Demonstrating cell culture on conductive polymer with the application of electric fields allows for the study of healthy and disease cell populations in the presence of electrical stimuli. Biomarker monitoring during this work is important to characterise and understand the impact of stimuli on the cells in culture. As such, an electrochemical miRNA biosensor was also explored in this work. The assay was based on the detection of miRNA through hydrogen peroxide degradation. The assay was built of screen-printed electrodes as a method to characterise cell cultures. The ability to monitor biomarkers both in vitro and in vivo is important in generating an understanding of disease models and in the development of point-of-care testing capabilities.
Metadata
Item Type:Thesis (PhD)
Date of Award:November 2023
Refereed:No
Supervisor(s):Forster, Robert and Cahill, Paul
Uncontrolled Keywords:bipolar electrochemistry; wireless electrochemistry, cardiomyocytes, miRNA
Subjects:DCU Subject Areas > Biological Sciences > Biosensors
DCU Subject Areas > Humanities > Biological Sciences > Biosensors
DCU Subject Areas > Biological Sciences > Biotechnology
DCU Subject Areas > Humanities > Biological Sciences > Biotechnology
DCU Subject Areas > Biological Sciences > Cell biology
DCU Subject Areas > Humanities > Biological Sciences > Cell biology
DCU Subject Areas > Biological Sciences > Microfluidics
DCU Subject Areas > Humanities > Biological Sciences > Microfluidics
DCU Subject Areas > Physical Sciences > Analytical chemistry
DCU Subject Areas > Physical Sciences > Chemistry
DCU Subject Areas > Physical Sciences > Electrochemistry
DCU Subject Areas > Physical Sciences > Nanotechnology
DCU Subject Areas > Physical Sciences > Thin films
DCU Faculties and Centres:DCU Faculties and Schools > Faculty of Science and Health > School of Chemical Sciences
Use License:This item is licensed under a Creative Commons Attribution-NonCommercial-No Derivative Works 4.0 License. View License
Funders:European Union INTERREG VA Programme, Eastern Corridor Medical Engineering Centre (ECME)
ID Code:28978
Deposited On:06 Nov 2023 15:19 by Robert Forster . Last Modified 06 Nov 2023 15:19
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