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Production and characterization of a polyclonal antibody fornext term Os(II) and Ru(II) polypyridyl complexes

O'Connor, Máire B., Killard, Anthony J. orcid logoORCID: 0000-0001-6953-3655, O'Kennedy, Richard, Forster, Robert J. and Smyth, Malcolm R. (2006) Production and characterization of a polyclonal antibody fornext term Os(II) and Ru(II) polypyridyl complexes. Journal of Inorganic Biochemistry, 100 (7). pp. 1252-1259. ISSN 0162-0134

Abstract
The characterization of a polyclonal antibody produced via immunization with an [Os(bpy)2dcbpy] hapten is described. Bpy is 2,2′-bipyridine and dcbpy is 2,2′-bipyridine-4,4′-dicarboxylic acid. The cross-reactivity of the antibody for the Ru(II) analogue of the hapten was also investigated. Large increases in the emission and luminescent lifetime of a series of Os and Ru complexes were observed on binding of the antibody. Association equilibrium constants were derived from luminescence titration data and were found to be 5.6 × 108 and 5.0 × 108 M−1 for [Os(bpy)2dcbpy] and [Ru(bpy)2dcbpy], respectively. Spectroscopic changes were likely due to the exclusion of H2O from the complex/antibody binding cleft and blocking of vibrational relaxation pathways of the Os/Ru excited state. D2O/H2O experiments confirmed that the antibody protected approx. 82% of [Os(bpy)2dcbpy] and 80% of [Ru(bpy)2dcbpy] from excited state deactivation by the aqueous solvent.
Metadata
Item Type:Article (Published)
Refereed:Yes
Uncontrolled Keywords:antibody; osmium(II); ruthenium(II); polypyridyl;
Subjects:Biological Sciences > Biotechnology
Biological Sciences > Biochemistry
Physical Sciences > Chemistry
DCU Faculties and Centres:DCU Faculties and Schools > Faculty of Science and Health > School of Chemical Sciences
DCU Faculties and Schools > Faculty of Science and Health > School of Biotechnology
Research Initiatives and Centres > National Centre for Sensor Research (NCSR)
Publisher:Elsevier
Official URL:http://dx.doi.org/10.1016/j.jinorgbio.2006.02.002
ID Code:49
Deposited On:08 Nov 2006 by DORAS Administrator . Last Modified 27 Sep 2019 11:22
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