Daly, Stephen J., Dillon, Paul P., Manning, Bernadette M., Dunne, Lynsey, Killard, Anthony J. and O'Kennedy, Richard (2002) Fv antibodies to aflatoxin B1 derived from a pre-immunized antibody phage display library system. Food and Agricultural Immunology, 14 (4). pp. 255-274. ISSN 1465-3443
Abstract
The production and characterization of recombinant antibodies to aflatoxin B[SUB1] (AFB[SUB1]), a potent mycotoxin and carcinogen is described. The antibody fragments produced were then applied for use in a surface plasmon resonance-based biosensor (BIAcore), which measures biomolecular interactions in 'real-time'. Single chain Fv (scFv) antibodies were generated to aflatoxin B1 from an established phage display system, which incorporated a range of different plasmids for efficient scFv expression. The scFv's were used in the development of a competitive ELISA, and also for the development of surface plasmon resonance (SPR)-based inhibition immunoassays. They were found to be suitable for the detection of AFB[SUB1], in this format, with the assays being sensitive and reproducible.
Metadata
| Item Type: | Article (Published) |
|---|---|
| Refereed: | Yes |
| Uncontrolled Keywords: | aflatoxin B1; single chain Fv; phage display technology; surface plasmon resonance; |
| Subjects: | Biological Sciences > Biotechnology Biological Sciences > Microbiology Biological Sciences > Biochemistry Physical Sciences > Chemistry |
| DCU Faculties and Centres: | DCU Faculties and Schools > Faculty of Science and Health > School of Chemical Sciences DCU Faculties and Schools > Faculty of Science and Health > School of Biotechnology Research Initiatives and Centres > National Centre for Sensor Research (NCSR) |
| Publisher: | Taylor & Francis |
| Official URL: | http://dx.doi.org/10.1080/0954010021000096373 |
| Use License: | This item is licensed under a Creative Commons Attribution-NonCommercial-Share Alike 3.0 License. View License |
| ID Code: | 47 |
| Deposited On: | 08 Nov 2006 by DORAS Administrator . Last Modified 19 Jul 2018 14:40 |
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