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Mediated amperometric immunosensing using single walled carbon nanotube forests

O'Connor, Máire B., Kim, Sang Nyon, Killard, Anthony J. orcid logoORCID: 0000-0001-6953-3655, Forster, Robert J., Smyth, Malcolm R., Papadimitrakopoulos, Fotios and Rusling, James F. (2004) Mediated amperometric immunosensing using single walled carbon nanotube forests. Analyst, 129 (12). pp. 1176-1180. ISSN 0003-2654

Abstract
A prototype amperometric immunosensor was evaluated based on the adsorption of antibodies onto perpendicularly oriented assemblies of single wall carbon nanotubes called SWNT forests. The forests were self-assembled from oxidatively shortened SWNTs onto Nafion/iron oxide coated pyrolytic graphite electrodes. The nanotube forests were characterized using atomic force microscopy and resonance Raman spectroscopy. Anti-biotin antibody strongly adsorbed to the SWNT forests. In the presence of a soluble mediator, the detection limit for horseradish peroxidase (HRP) labeled biotin was 2.5 pmol ml[-1] (2.5 nM). Unlabelled biotin was detected in a competitive approach with a detection limit of 16 nmol ml[-1] (16 μM) and a relative standard deviation of 12%. The immunosensor showed low non-specific adsorption of biotin-HRP (approx. 0.1%) when blocked with bovine serum albumin. This immunosensing approach using high surface area, patternable, conductive SWNT assemblies may eventually prove useful for nano-biosensing arrays.
Metadata
Item Type:Article (Published)
Refereed:Yes
Uncontrolled Keywords:enzyme; oxidoreductases; peroxidases; serum albumin; peroxidase; biotin; graphite; iron oxide; antibody; chemical enrichment; chemical sensor; biosensor; surface area; standard deviation; detection limit; raman spectrometry; atomic force microscopy; adsorption; prototype; carbon nanotubes; immunosensor; amperometry;
Subjects:Physical Sciences > Chemistry
DCU Faculties and Centres:DCU Faculties and Schools > Faculty of Science and Health > School of Chemical Sciences
Research Initiatives and Centres > National Centre for Sensor Research (NCSR)
Publisher:Royal Society of Chemistry
Official URL:http://dx.doi.org/10.1039/b412805b
Use License:This item is licensed under a Creative Commons Attribution-NonCommercial-Share Alike 3.0 License. View License
ID Code:39
Deposited On:02 Nov 2006 by DORAS Administrator . Last Modified 26 Oct 2018 09:28
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