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Laser nanostructured gold biosensor for proto-oncogene detection

Hughes, Cian orcid logoORCID: 0000-0002-4863-733X, Sreenilayam, Sithara orcid logoORCID: 0000-0002-5282-3645 and Brabazon, Dermot orcid logoORCID: 0000-0003-3214-6381 (2023) Laser nanostructured gold biosensor for proto-oncogene detection. Scientific Reports, 13 . ISSN 2045-2322

Abstract
The advancement of biosensor research has been a primary driving force in the continuing progress of modern medical science. While traditional nanofabrication methods have long been the foundation of biosensor research, recent years have seen a shift in the field of nanofabrication towards laser-based techniques. Here we report a gold-based biosensor, with a limit of detection (LoD) 3.18 µM, developed using environmentally friendly Laser Ablation Synthesis in Liquid (LASiS) and Confined Atmospheric Pulsed-laser (CAP) deposition techniques for the first time. The sensors were able detect a DNA fragment corresponding to the longest unpaired sequence of the c-Myc gene, indicating their potential for detecting such fragments in the ctDNA signature of various cancers. The LoD of the developed novel biosensor highlights its reliability and sensitivity as an analytical platform. The reproducibility of the sensor was examined via the production and testing of 200 sensors with the same fabrication methodology. This work offers a scalable, and green approach to fabricating viable biosensors capable of detecting clinically relevant oncogenic targets.
Metadata
Item Type:Article (Published)
Refereed:Yes
Additional Information:Article number: 17196
Subjects:Biological Sciences > Biochemistry
Biological Sciences > Biosensors
Engineering > Materials
Engineering > Biomedical engineering
Medical Sciences > Cancer
Physical Sciences > Electrochemistry
Physical Sciences > Lasers
Physical Sciences > Nanotechnology
Physical Sciences > Thin films
DCU Faculties and Centres:DCU Faculties and Schools > Faculty of Engineering and Computing > School of Mechanical and Manufacturing Engineering
Research Initiatives and Centres > I-Form
Publisher:Nature Portfolio
Official URL:https://doi.org/10.1038/s41598-023-44372-4
Copyright Information:© 2023 The Authors.
Funders:Science Foundation Ireland (SFI) under Grant Numbers 12/IA/1576, 16/RC/3872, 19/US-C2C/3579, European Regional Development Fund.
ID Code:29304
Deposited On:21 Dec 2023 15:15 by Cian Hughes . Last Modified 21 Dec 2023 15:15
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