Campylobacteriosis is a persistent pressure on health-care systems, with poultry being recognised as a key reservoir for Campylobacter spp. This study focused on the development of a novel, water-soluble, metallo-antimicrobial for use at farm level to reduce Campylobacter infection. Numerous water-soluble metallo-antimicrobials have been synthesised and characterised using fourier-transform infrared spectroscopy (FTIR) and ultraviolet (UV) spectroscopy. These bioactive complexes have shown a significant (p ≤ 0.05) capability to inhibit C. jejuni growth and significantly reduce (p ≤ 0.05) C. jejuni biofilm formation, however, analysis with scanning electron microscopy (SEM) showed that the product has no effect on C. jejuni biofilm morphology. Significant reductions (p ≤ 0.05) in attachment of C. jejuni to IPEC-J2 cells were shown in vitro. C. jejuni virulence gene expression was examined over a time-course using RT-qPCR. Upon exposure to Fe2(SO4)3 gluconate for 8 hours, the expression of genes related to attachment, invasion, toxicity, and iron uptake were observed to be significantly decreased (p ≤ 0.05) in comparison to the control group. Subsequently, a poultry study was conducted to test the effectiveness of the liquid bioactive iron complex in-vivo. This research showed Fe2(SO4)3 gluconate supplementation significantly increased (p ≤ 0.05) the final body weight of broilers. Shallow shotgun sequencing revealed the phylum Firmicutes was the most dominant phyla in the caecum of birds supplemented with Fe2(SO4)3 gluconate which translated to an increase in the relative abundance of bacteria species known to produce volatile fatty acids (VFAs). This finding was accompanied by an increase in the VFA concentrations within the caecum. Fe2(SO4)3 gluconate (40 ppm) supplemented birds also showed a significantly lower (p ≤ 0.05) level of potential zoonotic pathogens. These results illustrate that the use of metallo-antimicrobials offer a promising, and effective alternative for pre-harvest management of Campylobacter spp infections.