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Yeast mannan-rich fraction modulates endogenous reactive oxygen species generation and antibiotic sensitivity in resistant E. coli

Smith, Helen orcid logoORCID: 0000-0003-4861-0708, Grant, Sharon, Meleady, Paula orcid logoORCID: 0000-0001-5306-310X, Henry, Michael, O'Gorman, Donal orcid logoORCID: 0000-0002-8228-1488 and Clynes, Martin orcid logoORCID: 0000-0002-3093-8185 (2022) Yeast mannan-rich fraction modulates endogenous reactive oxygen species generation and antibiotic sensitivity in resistant E. coli. International Journal of Molecular Sciences, 24 (1). ISSN 1661-6596

Abstract
Mannan-rich fraction (MRF) isolated from Saccharomyces cerevisiae has been studied for its beneficial impact on animal intestinal health. Herein, we examined how MRF affected the formation of reactive oxygen species (ROS), impacting antibiotic susceptibility in resistant Escherichia coli through the modulation of bacterial metabolism. The role of MRF in effecting proteomic change was examined using a proteomics-based approach. The results showed that MRF, when combined with bactericidal antibiotic treatment, increased ROS production in resistant E. coli by 59.29 ± 4.03% compared to the control (p ≤ 0.05). We further examined the effect of MRF alone and in combination with antibiotic treatment on E. coli growth and explored how MRF potentiates bacterial susceptibility to antibiotics via proteomic changes in key metabolic pathways. Herein we demonstrated that MRF supplementation in the growth media of ampicillin-resistant E. coli had a significant impact on the normal translational control of the central metabolic pathways, including those involved in the glycolysis–TCA cycle (p ≤ 0.05)
Metadata
Item Type:Article (Published)
Refereed:Yes
Uncontrolled Keywords:antimicrobial resistance; yeast mannan fraction; bacterial metabolism; antibiotic susceptibility; reactive oxygen species
Subjects:UNSPECIFIED
DCU Faculties and Centres:DCU Faculties and Schools > Faculty of Science and Health > School of Biotechnology
Research Initiatives and Centres > National Institute for Cellular Biotechnology (NICB)
Publisher:MDPI
Official URL:https://dx.doi.org/10.3390/ijms24010218
Copyright Information:© 2022 The Authors
ID Code:28091
Deposited On:17 Feb 2023 14:43 by Thomas Murtagh . Last Modified 17 Feb 2023 14:43
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