Gaughran, Jennifer ORCID: 0000-0002-3659-036X, Kinahan, David J. ORCID: 0000-0003-1968-2016, Mishra, Rohit ORCID: 0000-0003-4279-4163 and Ducrée, Jens ORCID: 0000-0002-0366-1897 (2016) Solvent-selective membranes for automating sequential liquid release and routing of nucleic acid purification protocols on a simple spindle motor. In: 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, 9-13 Oct 2016, Dublin, Ireland.
Abstract
By incorporating a set of membranes which selectively dissolve upon contact with aqueous or or-ganic solvents at strategic locations on a disc cartridge, we succeeded to fully automate the solid-phase extraction of nucleic acids by varying the spin rate of a low-cost spindle motor. A solvent- and phase-selective graphene oxide (GO) membrane governs the routing of flows to designated elution and waste chambers. The serial release of on-board sample and reagents is centrifugo-pneumatically controlled by our previously introduced, event-triggered valving scheme. The entire process of ex-traction completes in less than 8 minutes.
Metadata
Item Type: | Conference or Workshop Item (Poster) |
---|---|
Event Type: | Conference |
Refereed: | Yes |
Uncontrolled Keywords: | Graphene oxide membranes; Automation; Nucleic acid purification |
Subjects: | Engineering > Biomedical engineering |
DCU Faculties and Centres: | DCU Faculties and Schools > Faculty of Science and Health > School of Physical Sciences Research Initiatives and Centres > National Centre for Sensor Research (NCSR) |
Use License: | This item is licensed under a Creative Commons Attribution-NonCommercial-Share Alike 3.0 License. View License |
Funders: | Programme for Research in Third Level Institutions (PRTLI) Cycle 5, European Regional Development Fund (ERDF) |
ID Code: | 21442 |
Deposited On: | 21 Oct 2016 08:08 by David Kinahan . Last Modified 01 Mar 2021 15:21 |
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