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A portable centrifugal analyser for liver function screening

Nwankire, Charles, Czugala, Monika, Burger, Robert, Fraser, Kevin J. orcid logoORCID: 0000-0002-9718-5405, O'Connell, Tríona M., Glennon, Thomas, Onwuliri, Blessing E., Nduaguibe, Isikaku E., Diamond, Dermot orcid logoORCID: 0000-0003-2944-4839 and Ducrée, Jens orcid logoORCID: 0000-0002-0366-1897 (2014) A portable centrifugal analyser for liver function screening. Biosensors and Bioelectronics, 56 . pp. 352-358. ISSN 1873-4235

Abstract
Mortality rates of up to 50% have been reported after liver failure due to drug-induced hepatotoxicity and certain viral infections(Gao et al. 2008). These adverse conditions frequently affect HIV and tuberculosis patients on regular medication in resource-poor settings. Here, we report full integration of sample preparation with read-out of a 5-parameter liver assay panel (LAP) on a portable, easy-to-use, fast and cost- efficient centrifugal microfluidic analysis system (CMAS). Our unique, dissolvable-film based centrifugo- pneumatic valving was employed to provide sample-to-answer fashion automation for plasma extraction (from finger-prick of blood), metering and aliquoting into separate reaction chambers for parallelized colorimetric quantification during rotation. The entire LAP completes in less than 20 minutes while using only a tenth the reagent volumes when compared with standard hospital laboratory tests. Accuracy of in-situ liver function screening was validated by 96 separate tests with an average coefficient of variance (CV) of 7.9% compared to benchtop and hospital lab tests. Unpaired two sample statistical t-tests were used to compare the means of CMAS and benchtop reader, on one hand; and CMAS and hospital tests on the other. The results demonstrate no statistical difference between the respective means with 94% and 92% certainty of equivalence, respectively. The portable platform thus saves significant time, labour and costs compared to established technologies, and therefore comply with typical restrictions on lab infrastructure, maintenance, operator skill and costs prevalent in many field clinics of the developing world. It has been successfully deployed in a centralised lab in Nigeria.
Metadata
Item Type:Article (Published)
Refereed:Yes
Uncontrolled Keywords:Liver assay panel; Centrifugal microfludics; Portable analyser; Dissolvable film valve
Subjects:Biological Sciences > Biochemistry
Biological Sciences > Microfluidics
Physical Sciences > Detectors
Computer Science > Software engineering
DCU Faculties and Centres:Research Initiatives and Centres > Biomedical Diagnostics Institute (BDI)
Research Initiatives and Centres > INSIGHT Centre for Data Analytics
Research Initiatives and Centres > National Centre for Sensor Research (NCSR)
Publisher:Elsevier
Official URL:http://dx.doi.org/10.1016/j.bios.2014.01.031
Copyright Information:© 2014 Elsevier
Use License:This item is licensed under a Creative Commons Attribution-NonCommercial-Share Alike 3.0 License. View License
Funders:Science Foundation Ireland under grant 10/CE/B1821, MC wishes to thank to the Marie Curie Initial Training Network funded by the EC FP7 People Program ATWARM (Marie Curie ITN, No. 238273), KJF acknowledges the Marie Curie Actions re-integration grant PIRG07-GA-2010-268365, DD wishes to acknowledge the Science Foundation Ireland under grant 07/CE/I1147
ID Code:19792
Deposited On:27 Mar 2014 13:53 by Kevin Fraser . Last Modified 10 Mar 2020 12:25
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