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Automated on-disc total RNA extraction from whole blood towards point-of-care for early-stage diagnostics

Dimov, Nikolay, Gaughran, Jennifer orcid logoORCID: 0000-0002-3659-036X, Clancy, Eoin, Barry, Thomas, Smith, J. Terry and Ducrée, Jens orcid logoORCID: 0000-0002-0366-1897 (2013) Automated on-disc total RNA extraction from whole blood towards point-of-care for early-stage diagnostics. In: The 17th Annual IEEE Conference on Solid-State Sensors, Actuators and Microsystems, 16-21 July, Barcelona, Spain.

Abstract
We present a novel integrated, centrifugo-pneumatic micro-homogenizer (“μHomogenizer”) for automated sample preparation and total RNA extraction from whole blood. Using a Trizol based protocol, this novel μHomogenizer efficiently lyses whole blood spiked with E. coli, retains the organic-mixed fraction and yields the aqueous phase with the total RNA content. By the interplay of microfluidic design and a protocol of rotational frequencies, we concatenate (and parallelize) a sequence of five subsequent liquid handling operations that complete in less than 10 minutes. A comparison of the total nucleotide content yields similar performance as conventional, essentially manual off-disc sample preparation methods.
Metadata
Item Type:Conference or Workshop Item (Paper)
Event Type:Conference
Refereed:Yes
Subjects:Biological Sciences > Microfluidics
Medical Sciences > Diseases
Engineering > Biomedical engineering
Biological Sciences > Biosensors
DCU Faculties and Centres:Research Initiatives and Centres > Biomedical Diagnostics Institute (BDI)
DCU Faculties and Schools > Faculty of Science and Health > School of Physical Sciences
Use License:This item is licensed under a Creative Commons Attribution-NonCommercial-Share Alike 3.0 License. View License
ID Code:19031
Deposited On:16 Aug 2013 11:04 by Nikolay Dimov . Last Modified 01 Mar 2021 15:26
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