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Polyaniline nanofibres as templates for the covalent immobilisation of biomolecules

Crean, Carol, Lahiff, Emer, Gilmartin, Niamh and Diamond, Dermot orcid logoORCID: 0000-0003-2944-4839 (2011) Polyaniline nanofibres as templates for the covalent immobilisation of biomolecules. Synthetic Metals, 161 (3-4). pp. 285-292. ISSN 0379-6779

Abstract
The attachment of antibodies onto polyaniline nanofibres using covalent chemistry was investigated for the first time. Polyaniline nanofibres were functionalised post-polymerisation to attach either amide or carboxylic acid side-groups. These templates could then be further modified to attach antibodies, specifically in this instance mouse immunoglobulin G (IgG). The resultant conjugates were characterised using a variety of techniques including infrared, UV–vis and Raman spectroscopy. Conjugates were then used to detect secondary antibodies (anti-IgG). Results from enzyme-linked immunoassay studies indi- cate successful binding of the antibody to the polyaniline nanofibres. Carboxyl functionalised polyaniline nanofibres are shown in particular to decrease non-specific binding in the immunoassay. Direct electri- cal communication between polyaniline nanofibres covalently linked to peroxidase-labelled antibodies was observed during cyclic voltammetry, which demonstrates their potential for further development as nano-dimensional immunosensors.
Metadata
Item Type:Article (Published)
Refereed:Yes
Uncontrolled Keywords:Polyaniline nanofibres; Conducting polymer functionalisation; Antibody conjugation; Immunoassay
Subjects:Engineering > Materials
Physical Sciences > Chemistry
DCU Faculties and Centres:DCU Faculties and Schools > Faculty of Science and Health > School of Biotechnology
Research Initiatives and Centres > National Centre for Sensor Research (NCSR)
Research Initiatives and Centres > CLARITY: The Centre for Sensor Web Technologies
Publisher:Elsevier
Official URL:http://dx.doi.org/10.1016/j.synthmet.2010.11.037
Copyright Information:© 2011 Elsevier
Use License:This item is licensed under a Creative Commons Attribution-NonCommercial-Share Alike 3.0 License. View License
Funders:Science Foundation Ireland under Grant No. 05/CE3/B754, EU Seventh Framework Programme for support in the form of a Marie Curie Re-Integration Grant No. PIRG02-GA-2007-224880, SFI 07/CE/I1147, Enterprise Ireland PC/2008/0149
ID Code:17890
Deposited On:09 Apr 2013 09:30 by Kevin Fraser . Last Modified 09 Jul 2019 13:40
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