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Protein immobilization onto poly(acrylic acid) functional macroporous PolyHIPE obtained by surface-initiated ARGET ATRP from multi-tasking amino-polyHIPE precursor.

Audouin, Fabrice, Larragy, Ruth, Fox, Mary, O'Connor, Brendan orcid logoORCID: 0000-0002-6857-1614 and Heise, Andreas orcid logoORCID: 0000-0001-5916-8500 (2012) Protein immobilization onto poly(acrylic acid) functional macroporous PolyHIPE obtained by surface-initiated ARGET ATRP from multi-tasking amino-polyHIPE precursor. Biomacromolecules, 13 (11). pp. 3787-3794. ISSN 1526-4602

Abstract
Amino-functional macroporous monoliths from polymerized high internal phase emulsion (polyHIPE) were surface modified with initiators for atom transfer radical polymerization (ATRP). The ATRP initiator groups on the polyHIPE surface were successfully used to initiate activator regeneration by electron transfer (ARGET) ATRP of (meth)acrylic monomers, such as methyl methacrylate (MMA) or tert-butyl acrylate (tBA) resulting in a dense coating of polymers on the polyHIPE surface. Addition of sacrificial initiator permitted control of the amount of polymer grafted onto the monolith surface. Subsequent removal of the tert- butyl protecting groups yielded highly functional polyHIPE-g-poly(acrylic acid). The versatility to use the high density of carboxylic acid groups for secondary reactions was demonstrated by the successful conjugation of enhanced green fluorescent protein (eGFP) and coral derived red fluorescent protein (DsRed) using EDC/sulfo-NHS chemistry, on the polymer 3D-scaffold surface. The materials and methodologies presented here are simple and robust, thus, opening new possibilities for the bioconjugation of highly porous polyHIPE for bioseparation applications.
Metadata
Item Type:Article (Published)
Refereed:Yes
Subjects:Physical Sciences > Analytical chemistry
Biological Sciences > Biotechnology
Biological Sciences > Molecular biology
DCU Faculties and Centres:DCU Faculties and Schools > Faculty of Science and Health > School of Chemical Sciences
DCU Faculties and Schools > Faculty of Science and Health > School of Biotechnology
Research Initiatives and Centres > Irish Separation Science Cluster (ISSC)
Publisher:American Chemical Society
Official URL:http://dx.doi.org/10.1021/bm301251r
Copyright Information:© 2012 ACS
Use License:This item is licensed under a Creative Commons Attribution-NonCommercial-Share Alike 3.0 License. View License
Funders:Science Foundation Ireland
ID Code:17795
Deposited On:28 Feb 2013 12:06 by Brendan O'connor . Last Modified 18 Oct 2018 12:37
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