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Affinity tagging & purification of the fucose binding LecB protein

Creavin, Aileen and O'Connor, Brendan orcid logoORCID: 0000-0002-6857-1614 (2007) Affinity tagging & purification of the fucose binding LecB protein. In: Centre for BioAnalytical Sciences meeting 2007, 10 Jun 2007, DCU, Dublin, Ireland.

Abstract
The fucose binding LecB protein is one of two identified lectins produced by the opportunistic pathogen Pseudomonas aeruginosa (PA01) and is implicated in contributing to its virulence. A large number of homologous proteins have been identified in other bacterial species that exhibit extremely high sequence identity and similarity to LecB. However, key amino acid residues known to participate in fucose binding in LecB are altered in many of these proteins. Some of these proteins have been shown to exhibit altered sugar specificities while others are as yet uncharacterised. The existence of such homologues suggests the sugar binding specificity of the LecB protein could potentially be further diversified through mutagenesis to generate novel biomolecular recognition molecules for glycoprotein characterisation and purification applications.
Metadata
Item Type:Conference or Workshop Item (Poster)
Event Type:Workshop
Refereed:Yes
Uncontrolled Keywords:Lectins; Pathogens
Subjects:Biological Sciences > Microbiology
Biological Sciences > Biochemistry
Biological Sciences > Molecular biology
DCU Faculties and Centres:DCU Faculties and Schools > Faculty of Science and Health > School of Biotechnology
Research Initiatives and Centres > Centre for Bioanalytical Science (CBAS)
Use License:This item is licensed under a Creative Commons Attribution-NonCommercial-Share Alike 3.0 License. View License
Funders:Enterprise Ireland
ID Code:17786
Deposited On:11 Feb 2013 14:33 by Brendan O'connor . Last Modified 18 Oct 2018 13:22
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